Adulteration of milk and detection
Authors
● Baruke Abdulabad (2019-dvmn-002), UVAS
● Umm-e-Habiba Siddique (2019-dvmn-005), UVAS
● Kainat naseer(2019-dvmn-006), UVAS
● Zainab zulfiqar(2019-dvmn-22), UVAS
● Aleeha naveed(2019-dvmn-026), UVAS
Milk
adulteration test
Introduction
From the earliest times and till now, milk is the food which can
be considered as the ideal food. Milk is having all the beneficial nutrients
that are required for the appropriate growth of adults and infants. It is an
unfortunate fact that milk is being adulterated throughout the world which is
continuously reducing the nutrients and benefits of milk. The term adulteration
describes the introduction of any harmful and other entities which can make
milk unsafe and misbranded. Different types of adulterants can be added in milk
such as starch, soap, table sugar and formalin etc. fortunately, there are many
test to judge the quality of milk that either it is adulterated or not.
Qualitative detection of adulteration is easy to do but the quantitative test
to judge milk adulteration is somewhat more complex.
Qualitative detection of milk adulterants
These tests are simple and easy to perform. These tests include
the simple color based techniques. The major fact about these techniques is
that these are backwards and valid for limited concentration at a time. These
techniques are not yet sufficiently précised. Qualitative detection; however,
are advantageous because these are very simple and easy to perform. In
adulteration, different chemicals are added to improve the taste of milk but
here are certain chemical which are very lethal and hazardous for life. These
chemical can lead to fatal death of individuals.
Quantitative test for adulteration detection
There are different was to identify the adulteration of milk
quantitatively. Some of them are mentioned below
Adulteration with foreign protein
Some of the consumers who have intolerance to lactose are given
the milk with some supplements such as soy, rice and other almond proteins. The
interesting fact is that the production cost of these supplements is actually
70% lower than the actual cost of milk. Some people take advantage of this fact
and intentionally add these supplements in milk to lower the total cost of milk
and to increase the profits.
Detection of these kinds of proteins in milk are polarimetric
techniques, Iso electric precipitation, SDS-PAGE, and HPLC method. NIR (Near Infra Red)
spectroscopy is now being used for the detection of powder present in milk.
Adulteration of pasteurized milk can be done by soya, pea and wheat proteins.
Some proteins present in milk which are having polyclonal antibodies are now
being detected by ELISA test.
Adulterated milk with different sources
The easiest and common way of milk adulteration is mixing the milk
of different species to increase the profits of sellers. This kind of
adulteration can be detected with certain tests. The fact of this detection is
that the quantitative detection of this kind of adulteration is more complex
because it contains the generic and non generic polymorphism. ICP-OES (Inductively
Coupled Plasma Emission Spectroscopy) is used for the detection of milk which
is being adulterated by the milk of different geographical origins. Milk adulteration in which cow milk is
adulterated with caprine milk is being tested by HPLC/ESI-MS (High Performance
Liquid Chromatography/Electrospray Ionization- Mass Spectroscopy).
Milk adulteration with urea
Urea is a natural content of milk but it is the major content of
non-protein nitrogen present in milk. There are two ways in which milk can be
adulterated with urea. These methods are described below
The addition of unspecified synthetic milk can be added to natural
milk. This adulteration can be detected by the use of infrared Raman spectroscopy technique.
GC/IDMS is the other test of testing the presence of urea in milk which is
quantitative detection.
Conclusion
The milk sellers are basically focusing on the profits and
financial gain things. They have nothing to do with the health of milk
consumers. They are intentionally adding different elements on natural milk to
lower the advantageous nutrients of milk. These supplements and chemicals can
lead to serious diseases and lead to fatal diseases in individual, awareness
and information is needed to get rid of this problem. There should be proper
legislation made for this act that if someone will find doing milk
adulteration, he will face the consequences. This awareness will be beneficial
in this case.
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